again: once more, a second (or further) time, repeating an action
This is the everyday meaning of the word and by far the most common. It simply marks that something happens over again, whether it is the next round of an action or a fresh repetition of it. Gospel narrative leans on it constantly to keep a scene moving: Jesus prays again, speaks again, asks again; a master sends servant after servant; a crowd shouts again.
The word does not say how many times something has happened before, only that this is another instance. Sometimes a writer pins the count down by adding a phrase like "a second time" or "a third time" alongside it, but the word on its own just means "once more."